RNA/cDNA Hybridization and Infectivity Tests Suggest that Barley Yellow Mosaic Virus Isolate M has a Bipartite Genome |
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Authors: | Dr R Koenig Dr W Huth |
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Institution: | Biologische Bundesanstalt, Institut für Viruskrankheiten der Pflanzen, Messeweg 11, D-3300 Braunschweig (W. Germany). |
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Abstract: | Northern blot analysis with random-primed cDNAs revealed that RNAs 1 and 2 of barley yellow mosaic virus (Ba YMV) isolate M have no extensive base sequence homologies. Both RNAs are needed for infection. RNA 2 is therefore neither a subgenomic nor a, satellite RNA, but rather an essential part of the viral genome. Ba YMV isolate M has thus a bipartite genome. |
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