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| CXCR4的抑制性多肽对乳腺癌细胞CXCR4和HER-2表达及HERCEPTIN药物敏感性的影响 | |
| 引用本文： | 陈昌杰,章菊,杨清玲,丁勇兴,李成华,王惠.CXCR4的抑制性多肽对乳腺癌细胞CXCR4和HER-2表达及HERCEPTIN药物敏感性的影响[J].中国组织化学与细胞化学杂志,2008,17(5). |
| 作者姓名： | 陈昌杰 章菊 杨清玲 丁勇兴 李成华 王惠 |
| 作者单位： | 1. 蚌埠医学院生化与分子生物学教研室 2. 蚌埠医学院临床检验诊断实验中心 3. 蚌埠市中心医院肿瘤科,安徽,233000 |
| 基金项目： | 安徽省自然科学基金，安徽省临床医学应用技术研究计划项目，蚌埠市科技计划项目 |
| 摘 要： | 目的 探讨CXCR4抑制性多肽对人乳腺癌细胞株SKBR3膜受体HER-2和CXCR4的蛋白表达及其对Herceptin药物敏感性的影响.方法 抑制性多肽、Herceptin单独或联合处理乳腺癌SKBR3细胞24、48h后,用MTT法观测SKBR3细胞的增殖抑制效应;Weatern blot和免疫纽化法检测SKBR3细胞中CXCR4和HER-2蛋白表达;RT-PCR检测HER-2、CXCR4 mRNA的表达,流式细胞仪检测细胞周期的变化.结果 多肽和Herceptin可不同程度地下调人乳腺癌细胞SKBR3中CXCR4和HER-2的蛋白表达.多肽对细胞生长抑制不明显,与Herceptin联合用药后,生长抑制率高于对照组和Herceptin单独用药组(P<0.001),48h生长抑制率为57.94%±5.3,且呈时问依赖(P<0.05),Herceptin单独作用SKBR3-细胞,细胞周期阻滞于G0/GI期,并且S期的比例减少,与多肽联合作用后,细胞周期又进一步阻滞于G2/M期,S期细胞进一步降低.结论 抑制性多肽能不同程度地下调膜受体HER-2和CXCR4的表达,提高人乳腺癌细胞株SKBR3对Herceptin药物的敏感性. |
| 关 键 词： | 抑制性多肽 Herceptin CXCR4 HRE2 乳腺肿瘤 |
THE EFFECT OF INHIBITORY POLYPEPTIDE OF CXCR4 ON THE EXPRESSION OF CXCR4 AND HER-2 OF BREAST CANCER CELLS AND THE SENSITIVITY OF HERCEPTIN |
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| Chen Changjie,Zhang Ju,Yang Qingling,Ding Yongxing,Li Chenhua,Wang Hui.THE EFFECT OF INHIBITORY POLYPEPTIDE OF CXCR4 ON THE EXPRESSION OF CXCR4 AND HER-2 OF BREAST CANCER CELLS AND THE SENSITIVITY OF HERCEPTIN[J].Chinese Journal of Histochemistry and Cytochemistry,2008,17(5). | |
| Authors: | Chen Changjie Zhang Ju Yang Qingling Ding Yongxing Li Chenhua Wang Hui |
| Institution: | Chen Changjie1,Zhang Ju2,Yang Qingling1,Ding Yongxing3,Li Chenhua3,Wang Hui2(1Department of Biochemistry & Molecular Biology,Bengbu Medical College,2Reseach Center of Clinical Laboratory Science,3Branch of Tumour of The Center Hospital of Bengbu,Anbui 233000,China) |
| Abstract: | Objective To study the effect of inhibitory polypeptide CXCR4 on human breast cancer cell line SKBR3 and observe the protein expression of membrane receptor HER-2 and CXCR4 and the effect of the sensitivity of SKBR3 to herceptin.Methods The breast cancer cells of SKBR3 were treated with inhibitory polypeptide and herceptin alone or together for 24h,48h.Western bloting and immunobistochemistry were used to detected the protein expression of CXCR4 and HER-2 in SKBR3 cells,The expression of HER-2 and CXCR4 mRN... |
| Keywords: | Herceptini CXCR4 HRE2 |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |