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| 大鼠局灶性脑缺血后缺血边缘区NG_2细胞的表达 | |
| 引用本文： | 高杰,张桂青,喻志源,王伟,谢敏杰. 大鼠局灶性脑缺血后缺血边缘区NG_2细胞的表达[J]. 中国组织化学与细胞化学杂志, 2010, 19(3): 225-228. DOI: 10.3870/zgzzhx.2010.03.004 |
| 作者姓名： | 高杰 张桂青 喻志源 王伟 谢敏杰 |
| 作者单位： | 1. 新疆石河子大学医学院护理系,石河子,832008;华中科技大学同济医学院附属同济医院神经内科,武汉,430030 2. 新疆石河子大学医学院护理系,石河子,832008 3. 华中科技大学同济医学院附属同济医院神经内科,武汉,430030 |
| 摘 要： | 目的观察局灶性脑缺血后缺血边缘区海马和皮层NG2细胞的动态表达,探讨其在脑缺血神经损伤与修复过程中所起的作用。方法将大鼠随机分为假手术组(sham)和脑缺血再灌注组,脑缺血再灌注组采用线栓法制备大鼠大脑中动脉阻塞再灌流模型(MCAO),假手术组不插入线栓,采用免疫荧光组织化学法结合共聚焦显微镜成像观察sham组及脑缺血后3d,7d,30d不同时间点缺血边缘区的海马CA1区和皮层区NG2的动态表达情况。结果脑缺血再灌注后缺血边缘区海马和皮层NG2胶质细胞表达增加,缺血后7d最明显。结论脑缺血后缺血边缘区存在NG2细胞的增生和形态变化可能与脑缺血后损伤修复密切相关。 |
| 关 键 词： | 脑缺血 缺血边缘区 NG2细胞 |
EXPRESSION OF NG2 CELLS IN ISCHEMIA BOUNDARY ZONE AFTER MCAO IN RATS |
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| Gao Jie,Zhang Guiqing,Yu Zhiyuan,Wang Wei,Xie Minjie. EXPRESSION OF NG2 CELLS IN ISCHEMIA BOUNDARY ZONE AFTER MCAO IN RATS[J]. Chinese Journal of Histochemistry and Cytochemistry, 2010, 19(3): 225-228. DOI: 10.3870/zgzzhx.2010.03.004 | |
| Authors: | Gao Jie Zhang Guiqing Yu Zhiyuan Wang Wei Xie Minjie |
| Affiliation: | 1Department of Nursing,School of Medicine,Shihezi University, Shihezi 832008, China; 2Department of Neurology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Techology, Wuhan 430030, China) |
| Abstract: | Objective To observe the expression of NG2 in ipsilateral hippocampus and cortex of rats after middle cerebral artery occlusion （MCAO）.Methods Rats were randomly divided into the sham-operated group and ischemia group.Focal cerebral ischemia in rats was induced by occluding the middle cerebral artery for 1 hour followed by repufusion.Immunofluorescent staining was used to show the expression o fNG2.Results MCAO induced the increases of NG2-positive cells in ipsilateral hippocampus and cortex, which peaked at day 7 after ischemia.Conclusion Proliferation of NG2 cells may play an active role in the process of neuronal damage and repair after focal ischemia. |
| Keywords: | Cerebral ischemia Boundary zone NG2 cell |
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