Proceedings: Freeze-drying of mycoplasma

The study shows the effect of protectants in the freeze-drying of Mycoplasma gallisepticum and M. synoviae, and the effect of the cultivation period required to prepare the bacterial suspension, on the survival of M. gallisepticum.Sucrose as a protectant is more effective in both Mycoplasmas. In detail, 12.5% sucrose produced the most satisfactory survival of M. gallisepticum by comparison with other protectants, and there was an optimal concentration. On the other hand, in M. synoviae higher survival than 90 % was obtained in 7.5%–17.5% sucrose.Survival of M. gallisepticum after freeze-dyring was affected by the growth stage of the organism at the time of preparation of the bacterial suspension.