参与在农杆菌介导遗传转化过程中的植物因子研究进展

随着农杆菌介导遗传转化过程中农杆菌一方转化机理的阐明,人们现在已经将目光转向了参与在农杆菌介导遗传转化过程中的植物因子。应用拟南芥突变体分析,酵母双杂交和cDNA的扩增片段长度多态性等技术人们已经部分阐明了参与在农杆菌向植物细胞的附着,TDNA的加工和转运,T链复合体向细胞核的转运,TDNA的整合等过程中的植物因子,并对植物基因对于农杆菌侵染的响应有了初步的了解。这些结果的获得不仅有助于人们加深对农杆菌介导遗传转化机理的认识而且有助于进一步扩大农杆菌介导遗传转化这一技术的应用范围 。

Progress of Study on plant factors that participated in Agrobacterium tumefaciens-mediated transformation

After elucidation the mechanisms of Agrobacterium tumefaciens itself when they infected plant cells,researchers have turned their interest to factors of plant at this process.Using Arabidopsis mutants,yeast two hybrid system and cDNA AFLP techniques,factors of plant cells participated in the attachment to themselves by Agrobacterium tumefaciens, processing and transferring of T DNA,transferring T complex into nucleus and the integration of T DNA into plant genome have been partially elucidated.These results not only can deepen our knowledge about Agrobacterium tumefaciens mediated transformation but also facilitate the using of this technique in a wider range.