An Integrated Genetic and Physical Map of the Autosomal Recessive Polycystic Kidney Disease Region |
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| Authors: | Xose M. Lens,Luiz F. Onuchic,Guanqing Wu,Tomohito Hayashi,Martin Daoust,Toshio Mochizuki,Lorenzo B. Santarina,John M. Stockwin,Gabi Mü cher,Jutta Becker,William E. Sweeny Jr.,Ellis D. Avner,Lisa Guay-Woodford,Klaus Zerres,Stefan Somlo,Gregory G. Germino |
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| Affiliation: | aDepartment of Medicine, Division of Nephrology, Johns Hopkins University School of Medicine, Baltimore, Maryland, 21205;bDepartment of Medicine and Department of Molecular Genetics, Division of Nephrology, Albert Einstein College of Medicine, Bronx, New York, 10461;cDepartment of Medicine and Pediatrics, University of Alabama at Birmingham, Birmingham, Alabama, 35294;dDepartment of Genetics, Bonn University, Bonn, D53111, Germany;eDepartment of Pediatrics, Rainbow Babies and Children's Hospital, Case Western Reserve University, Cleveland, Ohio, 44106 |
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| Abstract: | Autosomal recessive polycystic kidney disease is one of the most common hereditary renal cystic diseases in children. Genetic studies have recently assigned the only known locus for this disorder, PKHD1, to chromosome 6p21–p12. We have generated a YAC contig that spans 5 cM of this region, defined by the markers D6S1253–D6S295, and have mapped 43 sequence-tagged sites (STS) within this interval. This set includes 20 novel STSs, which define 12 unique positions in the region, and three ESTs. A minimal set of two YACs spans the segment D6S465–D6S466, which contains PKHD1, and estimates of their sizes based on information in public databases suggest that the size of the critical region is <3.1 Mb. Twenty-eight STSs map to this interval, giving an average STS density of <1/150 kb. These resources will be useful for establishing a complete trancription map of the PKHD1 region. |
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