云南省奶牛胚胎移植试验

实验用12头黑白花奶牛（3-9岁），PGF2α]作同步发情处理后，周期第10-12天，总剂量36 mg的FSH-P按递减方式每日肌肉注射2次，间隔12小时，共注射4天，在第3天加注PGF2α]35 mg，进行超数排卵处理。12头供体牛共得胚135枚，可用胚数118枚，为总胚数的87.47%。平均每头得胚11.25枚。胚胎分别移植给20头同步发情处理的黑白花奶牛和20头黄牛受体（有3头为自然发情），每头移植1枚胚胎。结果，奶牛受孕率为35%（受孕7头，产犊7头）；黄牛受孕率为40%（受孕8头，流产2头，产犊6头）。奶牛和黄牛总受孕率为37.5%。

THE STUDY OF BOVINE EMBRYO TRANSFER IN YUNNAN

12 donors (3 - 9 years old dairy cows) were superovulated with FSH-p twice daily in the dose of 36 mg for four days in 10-12th days of cycle after synchronized with PGF2a. At the third day of supcrovulation, additional 35 mg PGF2a were administrated intramuscularly. A total of 135 embryos were recovered 6-7 days after insermination. Of these embryos, 87.47% (118/135) showed good quality (average 9.8 embryos per donor). Particularly, we got 76 embryos from 3 donors (24, 26 and 27 respectively) and 69 of 76 were good quality (23 embryos per donor) . After transferred to synchronized recipients (20 dairy cows and 20 cattle) with total 40 embryos. 7 dairy cows (35%) and 8 cattle (40%) were pregnant. The average pregnant rate were 37.5%. A total of ]3 heifers were delivered to birth from 7 dairy cow and 6 cattle in this experiment.