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云南省奶牛胚胎移植试验
引用本文:陈云鹤,丁开荣.云南省奶牛胚胎移植试验[J].动物学研究,1991,12(4):381-385.
作者姓名:陈云鹤  丁开荣
作者单位:中国科学院昆明动物研究所 (陈云鹤,门红升,周瑆良,和协超),昆明市第二农场奶牛分场 (丁开荣,木桐先),云南省肉牛牧草研究中心(李天平)
摘    要:实验用12头黑白花奶牛(3-9岁),PGF2α]作同步发情处理后,周期第10-12天,总剂量36 mg的FSH-P按递减方式每日肌肉注射2次,间隔12小时,共注射4天,在第3天加注PGF2α]35 mg,进行超数排卵处理。12头供体牛共得胚135枚,可用胚数118枚,为总胚数的87.47%。平均每头得胚11.25枚。胚胎分别移植给20头同步发情处理的黑白花奶牛和20头黄牛受体(有3头为自然发情),每头移植1枚胚胎。结果,奶牛受孕率为35%(受孕7头,产犊7头);黄牛受孕率为40%(受孕8头,流产2头,产犊6头)。奶牛和黄牛总受孕率为37.5%。

关 键 词:奶牛  黄牛  胚胎移植  超数排卵

THE STUDY OF BOVINE EMBRYO TRANSFER IN YUNNAN
Chen Yunhe Men Hongsheng Zhou Xinliang He Xuechao.THE STUDY OF BOVINE EMBRYO TRANSFER IN YUNNAN[J].Zoological Research,1991,12(4):381-385.
Authors:Chen Yunhe Men Hongsheng Zhou Xinliang He Xuechao
Abstract:12 donors (3 - 9 years old dairy cows) were superovulated with FSH-p twice daily in the dose of 36 mg for four days in 10-12th days of cycle after synchronized with PGF2a. At the third day of supcrovulation, additional 35 mg PGF2a were administrated intramuscularly. A total of 135 embryos were recovered 6-7 days after insermination. Of these embryos, 87.47% (118/135) showed good quality (average 9.8 embryos per donor). Particularly, we got 76 embryos from 3 donors (24, 26 and 27 respectively) and 69 of 76 were good quality (23 embryos per donor) . After transferred to synchronized recipients (20 dairy cows and 20 cattle) with total 40 embryos. 7 dairy cows (35%) and 8 cattle (40%) were pregnant. The average pregnant rate were 37.5%. A total of ]3 heifers were delivered to birth from 7 dairy cow and 6 cattle in this experiment.
Keywords:Dairy cow  Cattle  Superovulation  Embryo transfer  
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