Mutagenesis studies on TenA: a thiamin salvage enzyme from Bacillus subtilis |
| |
Authors: | Jenkins Amy L Zhang Yang Ealick Steven E Begley Tadhg P |
| |
Institution: | Department of Chemistry and Chemical Biology, Cornell University, Ithaca, NY 14853, USA |
| |
Abstract: | TenA catalyzes the hydrolysis of 4-amino-5-aminomethyl-2-methylpyrimidine and participates in the salvage of base-degraded thiamin. Here, we describe mutagenesis of the active site of TenA guided by structures of the enzyme complexed to a substrate analog and to the product. Catalytic roles for each of the active site residues are identified and a mechanism for the reaction is described. |
| |
Keywords: | TenA Thiaminase II Mechanism Structure Mutagenesis Thiamin degradation |
本文献已被 ScienceDirect PubMed 等数据库收录! |