孕马血清促性腺激素和氯地酚对猕猴超数排卵效果的观察

用孕马血清促性腺激素,总剂量为1550—2000单位,分4—7天处理猕猴,可促使其每侧卵巢出现滤泡超数发育,随后静脉注射入绒毛膜促性腺激素2500单位,在24小时内,猕猴即可出现超数排卵。

THE EFFECT OF PMSG AND CLOMIPHENE ON SUPEROVULATION IN RHESUS MONKEY

Ten adult female rhesus monkeys, in condition without regard to their menstrual cycles, were divided into five groups and treated with PMSG or Clomiphene for 4 or 7 days. At 24 hours interval after the last injection, the. animals were treated with hCG. Ovarian and follicular morphology were observed by laparoscopy at prior to hormonal treatment, following 4th or 5th day and after the treatment of hCG, respectively. The blood samples were collected at the same time with laparoscopy for radioimmunoassay of .serum estrogen and progesterone levels. In the groups of treatment with 1260 IU PMSG and 100 mg or 160 mg Clomiphene, those ovaries couldn't be induced to poly-follicular development and ovulation. Their serum estrogen levels were below 220pg/ml. In the groups treated with 1550 IU PMSG and 1000 IU hCG, the ovaries were oedema obviously and several little uplifted reddish folliculi, with diameter 0.1-0.3 cm were seen on the surface of two ovaries. At 48 hr after treatment of 1000 IU hCG, the follicuii were increased markedly, with diameter to 0.3-0.5cm. The serum estrogen concentration reached 636.4 pg/ml, but hadn't ovulation. In the group of treatment with 2000 IU PMSG, the two ovaries of the animals occurred superovulation at 24hr after treatment with 2550 IU hCG.