Interleukin-2 inhibits NMDA receptor-mediated currents directly and may differentially affect subtypes |
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Authors: | Shen Yi Zhu Li-Jun Liu Shuang-Shuang Zhou Su-Ya Luo Jian-Hong |
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Institution: | Department of Neurobiology, Institute for Neuroscience, Zhejiang University School of Medicine, Hangzhou 310058, China. |
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Abstract: | Using whole-cell patch-clamp recordings, this study investigated the effects of interleukin-2 (IL-2) on N-methyl-d-aspartate (NMDA) receptor-mediated currents (I(NMDA)) in rat cultured hippocampal neurons and human embryonic kidney (HEK) 293 cells expressing recombinant NMDA receptors. We found that IL-2 (0.01-1ng/ml) immediately and significantly decreased peak I(NMDA) in cultured neurons. Interestingly, the peak I(NMDA) induced in HEK 293 cells was also inhibited by IL-2. We also found that IL-2 differentially decreased the peak amplitudes of NR2A- and NR2B-containing NMDA receptor-mediated currents (I(NR2A) and I(NR2B)) by 54+/-5% and 30+/-4%, respectively. These results provide new evidence that IL-2 induces rapid inhibition of peak currents of NMDA receptor-mediated responses with possible NR1/NR2A and NR1/NR2B subtype-differentiation, and suggest that the inhibition is mediated by direct interaction between IL-2 and NMDA receptors. |
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Keywords: | Rat Interleukin-2 NMDA receptors Hippocampal neuron Whole-cell patch clamp |
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