AFLP标记技术的发展和完善

AFLP标记技术自诞生以来,由于集成高效性、高通量、无需知道序列信息等诸多优点而在分子生物学研究中得以广泛应用。近年来,研究人员对该技术进行了不断的优化和完善,并由之衍生出多种相关技术,不仅能区分纯合子和杂合子,还能够将感兴趣AFLP条带进行位点特异性标记的高效转化,从而有利于进行大规模单位点检测和基因克隆,检测技术更趋高效。重点评述近年来“常规”AFLP技术向更高通量、更加高效和信息完全AFLP技术的转化和发展,了解这些变革对高效利用AFLP技术实现研究目标具有重要的意义。

The Advancement of AFLP Technology

AFLP technology has been widely used in molecular biology due to its integration of several advantages of high throughput,high efficiency and requiring no sequence information,etc. Great changes have been achieved in recent years in AFLP-related technologies and platforms. There are several AFLP-expanded technologies available. These improved technologies are capable of distinguishing the heterozygote from the homozygote and of converting any AFLP band of interest,without much effort,into locus-specific markers,which can be deployed for massive locus detection and for gene isolation. This review focuses on these favorable changes from conventional AFLP technology into more effective and more practicable AFLP-related ones. Understanding these advancements and AFLP-expanded technologies will facilitate the achievement of our research goals.