Measurement of urinary 3-methylhistidine with cationic-exchange resin |
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| Authors: | W L Fitch J E Watson J C King |
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| Affiliation: | 1. Eskisehir State Hospital, Department of Urology, Eskisehir, Turkey;2. Karabük University School of Medicine, Department of Urology, Karabük, Turkey;3. Eskisehir Osmangazi University Health Science Faculty Department of Nursing, Eskisehir, Turkey;4. Eskisehir State Hospital, Andrology Laboratory, Eskisehir, Turkey;1. Department of Applied Nutrition, Nutrition School, Federal University of the State of Rio de Janeiro, Rio de Janeiro, Brazil;2. Human Nutrition Research Unit, Department of Agricultural, Food and Nutritional Science, Division of Human Nutrition, University of Alberta, Edmonton, Alberta, Canada;3. Department of Applied Nutrition, Nutrition Institute, Rio de Janeiro State University, Rio de Janeiro, Brazil;1. Physiology of EXercise & Sport Lab, Department of Kinesiology, California State University Long Beach, Long Beach, CA, 90840, USA;2. Southern California University of Health Sciences, Whittier, CA, 90604, USA |
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| Abstract: | A simple method is presented for measurement of urinary 3-methylhistidine (3MH) using a cationic exchange resin treatment followed by colorimetric analysis. Equations are given to correct for the interference by histidine (4.3% by mole) in the colorimetric analysis. This correction is especially important for measurement of urinary 3MH in pregnant women or in other subjects with elevated histidine excretion. Good recovery of added standard and good reproducibility of results are documented. Preliminary data from a study of pregnant women are reported, suggesting an increased excretion of 3MH during pregnancy. Large day-to-day variability of 3MH excretion was observed within subjects. It is recommended that repeated measurements be done on each subject when determining 3MH excretion. |
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