Cartilage-derived factor (CDF) : II. Somatomedin-like action on cultured chondrocytes |
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| Authors: | Yukio Kato Yoshio Nomura Mitsuko Tsuji Hiroaki Ohmae Masahiko Kinoshita Shinji Hamamoto Fujio Suzuki |
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| Affiliation: | Department of Biochemistry and Calcified-Tissue Metabolism, Faculty of Dentistry, Osaka University, Nakanoshima, Kita-Ku, Osaka 530, Japan |
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| Abstract: | Previously, we showed that fetal bovine cartilage contains a polypeptide that stimulates the incorporation of [35S]sulfate into proteoglycans synthesized by rat and rabbit costal chondrocytes in culture. In this paper, we report that the cartilage-derived factor (CDF) increases not only [35S]sulfate incorporation but also [3H]thymidine incorporation into rabbit chondrocytes in monolayer culture. The dose-response curve of CDF stimulation of DNA synthesis was similar in profile to that of CDF stimulation of proteoglycan synthesis. In addition, CDF markedly enhanced [3H]uridine incorporation into rabbit chondrocytes and significantly enhanced [3H]serine incorporation into total protein. These findings indicate that fetal bovine cartilage contains a factor that shows somatomedin-like activity in monolayer cultures of rabbit chondrocytes. |
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| Keywords: | To whom offprint requests should be addressed. |
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