Reversal of alpha-difluoromethylornithine inhibition of caerulein-induced pancreatic growth by putrescine |
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Authors: | J Morisset O Benrezzak |
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Affiliation: | Centre de Recherche sur les Mécanismes de Sécrétion, Départment de Biologie - Faculté des Sciences, Université de Sherbrooke, Sherbrooke, Québec, Canada J1K 2R1 |
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Abstract: | The role of ornithine decarboxylase and of polyamines was investigated on caerulein-induced pancreatic growth through the use of alpha-difluoromethylornithine (DFMO) and putrescine. Caerulein, the cholecystokinin analog, given at a dose of 1 microgram . kg-1 three times a day was associated with pancreatic hyperplasia and hypertrophy after 2 and 4 days of treatment. The present study shows that putrescine, given once daily i.p. at a dose of 300 mumol . kg-1, can reverse the previously observed DFMO inhibition on pancreatic DNA content increments stimulated by caerulein. It was also observed that putrescine inhibits severely the 2-day caerulein-induced pancreatic hypertrophy, yet interferes only moderately with 4 days of caerulein treatment. These data lend further support to the involvement of ornithine decarboxylase and polyamines in induced pancreatic growth. |
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Keywords: | pancreatic growth caerulein putrescine DFMO DNA content and synthesis |
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