Tuning the expression level of recombinant proteins by modulating mRNA stability in a cell-free protein synthesis system |
| |
Authors: | Ahn Jin-Ho Kang Taek-Jin Kim Dong-Myung |
| |
Affiliation: | Institute of Molecular Biology and Genetics, Seoul National University, Seoul, Korea. |
| |
Abstract: | In this work, it was discovered that the stability of mRNA in a cell-free extract could be controlled by using engineered T7 terminator sequences. Specifically, it was found that mRNA stability gradually decreased as the length of the stem structure of the T7 terminator was reduced sequentially. As a result of the controlled abundance of mRNA species, it was possible to manipulate the relative expression level of target proteins by employing the T7 terminator of adjusted stem lengths. |
| |
Keywords: | cell‐free protein synthesis mRNA stability synthetic biology metabolic engineering tunable expression T7 terminator |
本文献已被 PubMed 等数据库收录! |