Qualitative and quantitative variation of tRNA in certain invertebrates |
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Authors: | P Sivaram R Mayasundari |
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Institution: | (1) Department of Genetics, Post-Graduate Institute of Basic Medical Sciences, Taramani, 600 113 Madras, India;(2) Present address: Department of Biochemistry, UConn Health Center, 06032 Farmington, Connecticut, USA |
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Abstract: | Total tRNA was isolated, purified and quantitated from earthworm, cockroach, fresh water mussel and rat liver. The total tRNA
content of invertebrates was found to be much lower than that of rat liver. When checked for aminoacylation capacity with
homologous and heterologous enzymes and algal protein hydrolysate, the tRNA preparation from rat liver and fresh water mussel,
a mollusc, were found to be active. On the other hand, the tRNAs from earthworm, an annelid, and cockroach, an arthropod,
were completely inactive with the homologous enzymes but showed partial activity with heterologous enzymes. Similar results
were obtained with individual amino acids also. The low activity or inactivity of earthworm and cockroach tRNAs appears to
be due to certain endogenous aminoacylation inhibitors.
This work was carried out at the School of Life Sciences, University of Hyderabad, Hyderabad 500 134, India. |
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Keywords: | Transfer RNA aminoacyl-tRNA synthetase aminoacylation earthworm cockroach fresh water mussel |
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