A mouse tool for conditional mutagenesis in ovarian granulosa cells |
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Authors: | Monica Musteanu Thomas Hoffmann Andreas Birbach Robert Eferl Emilio Casanova |
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Institution: | 1. Ludwig Boltzmann Institute for Cancer Research (LBI‐CR), A‐1090 Vienna, Austria;2. Department of Vascular Biology and Thrombosis Research, Medical University of Vienna, A‐1090 Vienna, Austria |
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Abstract: | Here we describe the generation of an inducible Cre transgenic line allowing conditional mutagenesis in ovarian granulosa cells. We have expressed the tamoxifen inducible CreERT2 fusion protein from a Bacterial Artificial Chromosome (BAC) containing the regulatory elements of the hydroxysteroid (17‐beta) dehydrogenase 1 (Hsd17b1) gene. Hsd17b1‐iCreERT2 transgenic mice express the iCreERT2 fusion protein exclusively in ovarian granulosa cells. Recombination analysis at the genomic DNA level using mice with “floxed” Stat3 alleles showed no Cre activity in absence of tamoxifen whereas tamoxifen treatment induced Cre activity solely in the ovaries. Further characterization of Hsd17b1‐iCreERT2 mice using a Cre reporter line demonstrated that Cre‐mediated recombination was restricted to ovarian granulosa cells. Therefore, Hsd17b1‐iCreERT2 mice should be a useful tool to analyze the gene functions in ovarian granulosa cells. genesis 48:612–617, 2010. © 2010 Wiley‐Liss, Inc. |
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Keywords: | iCreERT2 loxP tamoxifen Hsd17b1 eGFP Stat3 |
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