| 胃粘膜病变核仁组成区嗜银蛋白的银染计量和能谱分析 |
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| 引用本文： | 赵振金,徐根兴,余有训,喻方迩,陈进贵,张丽,张运芳.胃粘膜病变核仁组成区嗜银蛋白的银染计量和能谱分析[J].中国组织化学与细胞化学杂志,1993(1). |
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| 作者姓名： | 赵振金 徐根兴 余有训 喻方迩 陈进贵 张丽 张运芳 |
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| 作者单位： | 南京海军医学专科学校，南京海军医学专科学校，南京海军医学专科学校，南京海军医学专科学校，南京海军医学专科学校，江苏省人民医院，南京马群医院 南京 210049，南京 210049，南京 210049，南京 210049，南京 210049 |
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| 摘 要： | 对77例胃粘膜活检标本进行银染,显示核仁组成区相关的嗜银蛋白(Ag-NOR),进行计数和统计分析,并对银染标本作电镜观察及能谱分析。结果表明,Ag-NOR 颗粒计数的多少,对胃肿瘤恶性程度的诊断有重要参考价值。电镜证实银染物质定位于核仁组成区,银染阳性区能谱分析显示 Ag 峰,说明银染反应具特异性.
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| 关 键 词： | 胃癌 胃粘膜 银染 核仁组成区 |
SILVER STAINING MEASURE AND ENERGY SPECTRUM ANALYSIS ON ARGYOPHILIC PROTEIN IN THE NUCLEOLAR ORGANIZER REGIONS OF GASTRIC MUCOSAL LESION |
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| Zhao Zhenjin Xu Genxing Naval Medical College Nanjing,P.R.China.SILVER STAINING MEASURE AND ENERGY SPECTRUM ANALYSIS ON ARGYOPHILIC PROTEIN IN THE NUCLEOLAR ORGANIZER REGIONS OF GASTRIC MUCOSAL LESION[J].Chinese Journal of Histochemistry and Cytochemistry,1993(1). |
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| Authors: | Zhao Zhenjin Xu Genxing Naval Medical College Nanjing PRChina |
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| Institution: | Zhao Zhenjin Xu Genxing Naval Medical College Nanjing,210049,P.R.China |
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| Abstract: | A quantitative and statistical analysis has been made on the relative at- gyrophilic protein in the nucleolar organizer regions (NOR) of gastric mucosalbiopsy specimens of 77 cases by using the silver staining method.An electronmicroscopic observation and an energy spectrum analysis have also been madeon the silver stained specimen.The results showed that Ag-NOR granularcounts could be of great referent value to dignosing pernicious degree of gas-tric tumor;it was also proved by the electron microscopy that the silverstaining substances were located in the NOR,and an energy spectrum analysison the positive silver staining region indicated the Ag peak,which showedthe specificity for the silver staining reaction. |
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| Keywords: | Carcinoma of stomach Silver staining method Gastric mucosa Nucleolar organizer regions(NOR) |
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