Basic structural and functional characteristics of the epidermal barrier in wild mammals living in different habitats and climates |
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Authors: | Wilfried Meyer Judith Schmidt Johannes Kacza Roger Busche Hassan Y Naim Ralf Jacob |
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Institution: | (1) Institute for Anatomy, University of Veterinary Medicine Hannover, Foundation, Bischofsholer Damm 15, 30173 Hannover, Germany;(2) Institute of Veterinary Anatomy, University of Leipzig, 04103 Leipzig, Germany;(3) Institute for Physiological Chemistry, University of Veterinary Medicine Hannover, Foundation, 30559 Hannover, Germany;(4) Institute of Cytobiology and Cytopathology, University of Marburg, 35033 Marburg, Germany |
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Abstract: | Based on the combination of standard light and transmission electron microscopy, cryo-SEM, immunohistochemistry and a new
sensitive glycolipid histochemical technique (5-hexadecanoylaminofluorescein staining, laser scanning microscopy), including
densitometrical evaluation, our approach gives for the first time an overview of the specific biology of the epidermal permeability
barrier in wild mammals (20 species from five orders), living under varying (aquatic or moist to dry) habitat conditions.
The results obtained emphasised that the barrier region in most of the species studied is a continuous zone (thickness, 0.1
and 3 μm) between the upper cells of the stratum granulosum and the inner cells of the stratum corneum conjunctum, normally
present as a homogeneous glycolipid layer originating from fusion of lamellar body contents after exocytotic activities of
the granular cells. However, this finding did not apply to all of the species studied, i.e., the Wild boar, the Common seal
and the three large species with a very thick vital epidermis, the African elephant, the hippopotamus and the common dolphin,
exhibited variations from the basic scheme. Densitometric evaluation of the 5-hexadecanoylaminofluorescein staining revealed
that reaction intensity was not only generally related to the habitat conditions but also to vital epidermis thickness and
hair density. The immunohistochemical demonstration of Na+/H+ exchanger 1 corroborated for all wild mammals studied that this important regulator of pH conditions during barrier formation
is continuously produced in the epidermis. The variations in barrier biology observed for some species obviously had to be
developed in relation to animal size (or body size area) and hair coat density, but, particularly, by the specific adaptation
of certain mammalian groups to the aquatic environment. In the latter case, the typical barrier zone system was lost, as in
the hippopotamus or the cetaceans. |
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