Citrate permease gene expression in Lactococcus lactis subsp. lactis strains IL1403 and MG1363 |
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Authors: | Gerald Bourel,Sadjia Bekal,Charles Diviè s,Hervé Pré vost |
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Affiliation: | Laboratoire de Microbiologie, ENSBANA, Universitéde Bourgogne, 1-Esplanade Erasme, 21000 Dijon, France |
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Abstract: | Abstract Citrate permease gene expression in the plasmid-free Lactococcus lactis strains IL1403 and MG1363 was studied. The ability to transport citrate results in diacetyl and acetoin production in IL1403 but not in MG1363. Citrate lyase, α-acetolactate decarboxylase, diacetyl and acetoin reductase were detected in IL1403. These data show that L. lactis ssp. lactis strain IL1403 is a citrate permease mutant of the biovar. diacetylactis . Immunological analysis revealed the α-and β-subunits of citrate lyase not only in IL1403 but also in MG1363 where no citrate lyase activity was found. |
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Keywords: | Lactococcus lactis IL1403 MG1363 Citrate permease Citrate lyase Diacetyl |
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