靶向敏感免疫脂质体药物释放机理的初步研究

本文利用浊度测量和~(31)P核磁共振(~(31)P-NMR)技术研究了靶向敏感兔疫脂质体(targea-sensitive immunoiposomes,TSIL)内含物的释放途径,结果表明药物穿膜释放与脂的多型性即由脂双层转变为非双层H_(11)相有关.

PRELIMINARY STUDIES ON RELEASE MECHANISM OF DRUG ENTRAPPED IN TARGET-SENSITIVE IMMUNOLIPOSOME (TSIL)

TSIL is composed of DOPE and anti-IgG coupled palmitic acid (P-anti-IgG). The size of TSIL is about 100nm which was observed at electron microscope by negative stain. The turbidity of TSIL increases as adding IgG, indicating that TSIL aggregation occurrse by interaction of TSIL with IgG or fusion between TSIL. 31P-NMR spectra of TSIL show that lipids adopt bilayer phase and the structure in which the lipid molecules undergo rapid isotropic motion. Addition of IgG resulted in the formation of hexagonal H11 phase, and the molecules of rapid isotropic motion were unchanged. The formation of non-bilayer H11 phase may be described as a mechanism of drng release entrapped in TSIL.