温度及膜中蛋白质含量与膜粘度和膜中分子排列方向的关系

本实验用闪光诱导的瞬间二向色性方法测量了不同温度以及不同蛋白质含量下菌紫质分子在脂质囊泡膜中的旋转扩散运动.根据旋转扩散运动得到了温度和蛋白质含量与膜粘度以及分子在膜中排列方向的关系.温度和蛋白质的含量都影响膜的粘度,但并不影响蛋白质分子在膜中的排列方向.

EFFECT OF TEMPERATURE AND PROTEIN CONCENTRATION IN MEMBRANE ON THE MEMBRANE VISCOSITY AND ORIENTAION OF MOLECULES IN MEMBRANE

The rotational diffusion of bacteriorhodopsin at different temperature and different amount of protein in membrane were measured by the technique of flash-induced transient dichroism. We observed the effects of temperature and amount of protein in the membrane on the viscosity of the membrane and the orientation of bacteriorhodopsin. Temperature and the amount of protein in the membrane affect viscosity of the membrane, but no effect on orientaton of bacteriorhodopsin in the membrane.