Breakdown of phosphatidylinositol in soybean callus |
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| Authors: | R. J. A. Connett D. E. Hanke |
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| Affiliation: | (1) Botany School, University of Cambridge, Downing Street, CB2 3EA Cambridge, UK;(2) Present address: Unit 154/155, Agricultural Genetics Company, Cambridge Science Park, CB4 4BH Cambridge, UK |
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| Abstract: | We have investigated the breakdown of membrane-bound phosphatidylinositol (PI) in homogenates of soybean (Glycine max) callus. The breakdown of PI was stimulated by the detergent deoxycholate. At pH 7.0 and 1·gl-1 of deoxycholate the loss of PI was rapid and extensive: more than 80% was broken down within 10 min. The breakdown of PI was also stimulated by millimolar concentrations of Ca2+. The products of breakdown of added PI (purified from soybean callus) in this system were identified from their chromatographic mobilities as 1,2-diacylglycerol, myo-inositol 1-phosphate and myo-inositol 1:2-cyclic monophosphate.Abbreviations DOC deoxycholate - EDTA ethylenedi-aminetetraacetic acid,-acetate - Pi Inorganic phosphate - PI phosphatidylinositol - PS phosphatidylserine - TLC thinlayer chromatography |
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| Keywords: | Callus 1,2-Diacylglycerol Glycine (phosphatidylinositol) Inositol phosphates Phosphatidylinositol Phospholipase C |
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