Cytochemical studies on Endamoeba histolytica,with particular reference to polysaccharides |
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| Authors: | HALLMAN F A MICHAELSON J B BLUMENTHAL H DELAMATER J N |
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| Affiliation: | 1. Departments of Medical Microbiology, Biochemistry and Medicine, University of Southern California School of Medicine, Los Angeles, USA;2. The Institute of Medical Research, Huntington Memorial Hospital, Pasadena, California, USA;1. Department of Physiology, University of Pretoria, Pretoria 0001, South Africa;2. Department of Medicine III & Center for Healthy Aging, Technische Universität Dresden, 01307, Germany;3. School of Food and Nutrition, Massey Institute of Food Science and Technology, Massey University, Palmerston North 4442, New Zealand;4. Institute for Food, Nutrition and Well-being, University of Pretoria, Pretoria 0001, South Africa;5. Department of Human Nutrition, University of Pretoria, Pretoria, South Africa;1. U.S. Department of Agriculture, Agricultural Research Service, Beltsville Human Nutrition Research Center, Beltsville, MD 20705, USA;2. Department of Food Science and Human Nutrition, University of Illinois, Urbana, IL 61801, USA;3. U.S. Department of Agriculture, Agricultural Research Service, Statistics Group, Beltsville, MD 20705, USA;4. National Institutes of Health, National Cancer Institute, Division of Cancer Protection, Rockville, MD 20852, USA;1. University of Belgrade, Faculty of Biology, Studentski trg 16, Belgrade, Serbia;2. University of Belgrade, Faculty of Medicine, Doktora Subotića 8, Belgrade, Serbia;3. University of Belgrade, Institute for Biological Research “Siniša Stanković”, Bulevar despota Stefana 142, Belgrade, Serbia;4. University of Belgrade, Faculty of Pharmacy, Vojvode Stepe 450, Belgrade, Serbia;5. University Medical Centre “Bežanijska kosa”, Bežanijska kosa bb, Belgrade, Serbia;1. Guelph Research and Development Centre, Agriculture and Agri-Food Canada, Guelph, ON N1G 5C9, Canada;2. Department of Human Health and Nutritional Sciences, University of Guelph, Guelph, ON N1G 2W1, Canada;3. Department of Physiology and Biochemistry of Nutrition, Max Rubner-Institute, Karlsruhe, Germany;4. School of Nutrition Sciences, University of Ottawa, Ottawa, ON K1H 8L1, Canada |
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| Abstract: | Cytochemical staining reactions indicate that cultured amebae and amebae from tissue contain polysaccharides.The effect of prior exposure to beta-glucuronidase and to saliva on the staining reaction of amebae from cultures containing starch suggests that more than one type of polysaccharide may exist in these cells. The lack of activity of these enzymatic substances on the staining reaction of amebae from starch-free cultures and from tissues would indicate that the polysaccharide content of amebae varies under different conditions of nutrition.When stained by Toluidine blue O, metachromasia is slight or absent in amebae from starch cultures and in those from liver tissue, but is marked in amebae from starch-free cultures. |
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