| 薯蓣皂甙元ELISA定量分析方法的建立Ⅰ-薯蓣皂甙元全抗原的合成 |
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| 引用本文： | 杨红艳,袁丽红,吴红利,于潇,欧阳平凯.薯蓣皂甙元ELISA定量分析方法的建立Ⅰ-薯蓣皂甙元全抗原的合成[J].天然产物研究与开发,2006,18(2):254-256. |
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| 作者姓名： | 杨红艳 袁丽红 吴红利 于潇 欧阳平凯 |
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| 作者单位： | 南京工业大学制药与生命科学学院,南京,210009 |
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| 摘 要： | 建立薯蓣皂甙元的ELISA定量分析方法必须先合成薯蓣皂甙元的全抗原。试验利用薯蓣皂甙元3位上的-OH,在DMAP的催化下,薯蓣皂甙元与丁二酸酐反应,生成薯蓣皂甙元丁二酸单酯,用MSI、R1、H NMR1、3CNMR等方法对产物结构进行了表征。用混合酸酐法制备薯蓣皂甙元与牛血清白蛋白的结合物(DG-HS-BSA),碳二亚胺法制备薯蓣皂甙元与卵清蛋白的结合物(DG-HS-OVA),经TNBS测定,每分子结合物连接的薯蓣皂甙元分子数分别为28.0和8.8。
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| 关 键 词： | 薯蓣皂甙元丁二酸单酯 薯蓣皂甙元 牛血清白蛋白 卵清蛋白 |
| 文章编号： | 1001-6880（2006）02-0254-03 |
| 收稿时间： | 2005-04-04 |
| 修稿时间： | 2005-07-04 |
Synthesis of Diosgenin-BSA and Diosgenin-OVA Conjugates |
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| YANG Hong-yan,YUAN Li-hong,WU Hong-li,YU Xiao,OUYANG Ping-kai.Synthesis of Diosgenin-BSA and Diosgenin-OVA Conjugates[J].Natural Product Research and Development,2006,18(2):254-256. |
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| Authors: | YANG Hong-yan YUAN Li-hong WU Hong-li YU Xiao OUYANG Ping-kai |
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| Institution: | College of Life Science and Pharmaceutical Engineering, Nanjing Univesity of Technology, Nanjing 210009, China |
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| Abstract: | An enzyme-linked immunosorbent assay(ELISA)to detect and quantify diosgenin in cell or tissue cultures is being developed.In the presence of succinic anhydride and catalyst DMAP,diosgenin was derivatized into diosgenin hemisuccinate,with reactive carboxyl group.The product was characterized by MS,IR,~1HNMR and()~(13)C-NMR spectral data.Diosgenin hemisuccinate was coupled with BSA by mixed anhydride method and ovalbumin by method of water-soluble carbodiimide.The number of diosgenin residues per molecule of the conjugates was 28.0 and 8.8,respectively.The successful preparation of diosgenin-protein conjugates made it possible to quantify diosgenin by ELISA. |
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| Keywords: | Diosgenin hemisuccinate diosgenin BSA ovalbumin |
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