Application of two-dimensional electrophoresis for monitoring gastrointestinal digestion of milk |
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Authors: | M Carbonaro |
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Institution: | (1) Istituto Nazionale di Ricerca per gli Alimenti e la Nutrizione, Rome, Italy |
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Abstract: | Summary. Two-dimensional electrophoresis (2-DE) was used for tracing in vivo gastrointestinal digestion of milk proteins in a rapid
model system with rats. Contents of stomach and small intestine from digestion trials with rats given a single dose of milk
powder were recovered after 1 hour. They were then subjected to 2-DE (IEF and SDS-PAGE). 2-DE showed undigested proteins in
a MW range 13.0–66.0 kDa in stomach and 13.0–25.0 kDa in the small intestine, thus indicating that milk proteins are slowly
digested. This approach may shed light on pattern of protein digestion and mechanism of amino acid and peptide assimilation. |
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Keywords: | : 2-DE – In vivo digestion – Milk |
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