Effect of glutathione on UV induction of prophage lambda |
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Authors: | Yancheng?Liu Qiuju?Zhang Email author" target="_blank">Chengxiang?FangEmail author Shiqiao?Zhu Yali?Tang Shaoyi?Huang |
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Institution: | (1) College of Life Sciences, Wuhan University, Wuhan, 430072, People’s Republic of China |
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Abstract: | The effect of glutathione (GSH) on the ultraviolet (UV) induction of lambda prophage was investigated in lysogenic Escherichia coli. The data showed that extracellular GSH could inhibit the UV induction of lambda prophage. The inhibitory rates were concentration
dependent, and the maximal rate obtained was 94% with 3.0 M GSH. The effect was also measured in three different lambda lysogens:
a wild-type strain (wt), an isogenic GSH-deficient strain, and an isogenic strain producing increased amounts of GSH. The
result showed that when subjected to UV irradiation (254 nm, 60 J m−2), GSH-deficient strain was approximately fivefold more sensitive to be lysed than wt, whereas the strain with higher intracellular
GSH levels was only 28% susceptible to be lysed. With electron spin resonance and spin trapping techniques, we observed that
free radical signals occurred in the suspensions of UV irradiated lysogenic cells and the intensity of signals was influenced
by GSH levels. These results indicate that GSH can significantly inhibit the UV induction of lambda prophage, and that this
effect is correlated to its capacity to scavenge free radicals generated after UV irradiation. |
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Keywords: | Glutathione Lambda prophage UV induction Free radical |
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