Pseudomonas cepacia lipase: Esterification reactions in AOT microemulsion systems |
| |
| Authors: | H Stamatis A Xenakis U Bornscheuer T Scheper U Menge F N Kolisis |
| |
| Institution: | (1) Institute of Biological Research & Biotechnology, National Hellenic Research Foundation, 48, Vas. Constantinou Ave., 116 35 Athens, Greece;(2) Biosystems Technology Laboratory, Chemical Engineering Dept., Division IV, National Technical University of Athens, Zographou campus, 157 00 Athens, Greece;(3) Institut fur Technische Chemie, Callinstr. 3, 3000 Hannover 1, Germany;(4) Gesellschaft fur Biotechnologische Forsung, Mascheroder Weg 1, 3000 Braunschweig, Germany |
| |
| Abstract: | Summary The activity of purifiedPseudomonas cepacia lipase has been investigated in esterification reactions of various aliphatic alcohols with natural fatty acids. The reactions were carried out in microemulsions formed in isooctane by bis(2ethylhexyl)sulfosuccinate sodium salt (AOT). The optima pH, T and water content (wo) for the enzyme activity in this type of microemulsions have been determined. Studies on the effect of various fatty acids and alcohols on the enzyme specificity have shown a preference of this lipase for palmitic and caprylic acid as well as for propanol, while reactions involving cyclic alcohols can not be catalyzed at all. The differences on the behavior of this lipase as compared to other lipases studied in microemulsion systems as well as in other systems are discussed. |
| |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ |
|
