Gas chromatographic measurement of urinary 5-fluoro-2'-deoxyuridine levels after barium salt precipitation and sephadex LH-20 cleanup |
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Authors: | C F Gelijkens A P De Leenheer |
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Institution: | Institut für Biochemie d. Johannes Gutenberg-Universität, Joh. Joachim Becher-Weg 30, D-6500 MainzWest Germany. |
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Abstract: | A fluorescent photoaffinity label—8-azido-1-N6-etheno-adenosine 5′-triphosphate (8-N3ε ATP)—for ATP-binding proteins has been synthesized. The effectiveness of the label is demonstrated with F1ATPase from Micrococcus luteus. 8-N3ε ATP is a substrate for the enzyme in the presence of bivalent cations. Ultraviolet irradiation of F1ATPase in the presence of the label and Mg2+ ions inhibits the enzyme irreversibly. The fluorescent label is bound preferentially to the β subunit of the enzyme. Labeling and inactivation are decreased by protection with ATP or ADP. |
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Keywords: | To whom all correspondence should be addressed |
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