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Fluorescence polarization in studies of bacterial cytoplasmic membrane fluidity under environmental stress |
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| Authors: | Mykytczuk N C S Trevors J T Leduc L G Ferroni G D |
| Affiliation: | aDepartment of Biology, Laurentian University, Sudbury, Ont., Canada P3E 2C6 bDepartment of Environmental Biology, University of Guelph, Ont., Canada N1G 2W1 cNorthern Ontario School of Medicine, Laurentian Campus, Sudbury, Ont., Canada 2C6 |
| Abstract: | The integrity of the bacterial cytoplasmic membrane is critical in maintaining the viability of cells and their metabolic functions, particularly under stress. Bacteria actively adjust membrane fluidity through changes in lipid composition in response to variations in temperature, pressure, ion concentrations, pH, nutrient availability, and xenobiotics. Fluorescence polarization methods are valuable for measuring bacterial cytoplasmic membrane fluidity. In this review we discuss the mechanisms of bacterial membrane adaptations and present data from research using 1,6-diphenyl-1,3,5-hexatirene (DPH) as a measure of membrane fluidity and phase transitions. We illustrate the range of fluidity in viable cells, extracted membranes, and liposomes under optimal and stressed physiological conditions. |
| Keywords: | Bacteria Cell Cytoplasmic membrane DPH Environment Fluidity Fluorescent probes Lipids Polarization Stress Spectrofluorometer Survival |
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