Effects of serotonin,dopamine and prostaglandin E2 on adenylate cyclase activity and cyclic AMP levels in different ganglia of the freshwater snail Planorbis corneus L. |
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| Institution: | 1. Department of Traditional Medicine, Wan Fang Hospital, Taipei Medical University, Taipei 116, Taiwan;2. Ph.D. Program for the Clinical Drug Discovery from Botanical Herbs, College of Pharmacy, Taipei Medical University, Taipei 110, Taiwan;3. Graduate Institute of Pharmacognosy, College of Pharmacy, Taipei Medical University, Taipei 110, Taiwan;4. National Research Institute of Chinese Medicine, Ministry of Health and Welfare, Taipei 112, Taiwan;5. Center for Reproductive Medicine & Sciences, Taipei Medical University Hospital, Taipei 110, Taiwan;1. USP - Department of Pharmacy, School of Pharmaceutical Sciences, University of Sao Paulo, Sao Paulo, Brazil;2. UNIFESP - Department of Pharmaceutical Sciences, Universidade Federal de São Paulo, Diadema, Brazil;3. UNIFESP – Department of Chemistry, Universidade Federal de São Paulo, Diadema, Brazil;4. Therapeutics Research Centre, The University of Queensland School of Medicine, Brisbane, Australia;5. IPEN/CNEN - Energy Research Institute, São Paulo, Brazil |
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| Abstract: | The effects of different neuroactive agents on cyclic AMP level of selected ganglia of Planorbis corneus were studied. Serotonin, dopamine and prostaglandin E2 were capable of increasing significantly cyclic AMP synthesis in all the preparations. When such substances were tested in pairs, supra-additive effects were always observed. In high Ca2+-high Mg2+ solutions dopamine action was blocked, meanwhile serotonin and prostaglandin E2 were still effective in stimulating cyclic AMP synthesis. In the same experimental condition the supra-additive increases of the nucleotide level by drug combinations disappeared. Serotonin, but not dopamine, significantly stimulated adenylate cyclase activity in all the preparations, while prostaglandin E2 was effective only in the Viscero-Parietal Complex. The presence of the adenylate cyclase activity in the nervous tissue of Planaorbis was substained by histochemical studies.These results demonstrating that in the nervous system of Planorbis cyclic AMP level is affected by neurotransmitters and neuromodulators, might support the idea of the crucial role of the cyclic nuclotide in the modulation of synaptic transmission. |
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