Isolation and characterization of biochemical and morphological mutants in chlamydomonas smithii |
| |
| Institution: | 1. College of Science, Nanjing Agricultural University, Nanjing 210095, China;2. College of Science, Nanjing Forestry University, Nanjing 210037, China;3. College of Agriculture, Nanjing Agricultural University, Nanjing 210095, China;1. Plant Science Division, Research School of Biology, 134 Linnaeus Way, Australian National University, Acton, ACT 2601, Australia;2. Research School of Chemistry, 137 Sullivan’s Ck Rd, Australian National University, Acton, ACT 2601, Australia;3. Discipline of Biological Sciences, School of Environmental and Life Sciences, University Drive, The University of Newcastle, Callaghan, NSW 2308, Australia;1. State Key Laboratory of Materials-Oriented Chemical Engineering, College of Food Science and Light Industry, Nanjing Tech University, Nanjing 211816, PR China;2. College of Biological and Food Engineering, Changshu Institute of Technology, 99 South Third Ring Road, Changshu 215500, PR China;3. Nanjing Shineking Biotech Co. Ltd., Nanjing 210061, PR China |
| |
| Abstract: | Mutants of Chlamydomanas smithii resistant to chlorate were induced after UV irradiation. Some were unable to grow on nitrate and suspected to be defective in nitrate reductase. One of them (nit-a) was analyzed and shown to complement nit-1 nit-2 of C. reinhardtii.Mutants resistant to streptomycin, spectinomycin or erythromycin selected from nit-a pre-grown in the presence of 5-fluorodeoxyridine. Two spectinomycin resistant strains were genetically analyzed from crosses with C. reinhardtii carrying different chloroplast mutations. The transmission of resistance was shown to be non-Mendelian and similar to that obtained in intraspecific crosses involving C. reinhardtii chloroplast mutants.Mutants lacking a cell wall (CW−) were selected after UV treatment on the basis of the particular flat amoeboid shape of the colonies. Seven of them were tested for complementation with CW15 and CWd mutants of C. reinhardtii: none was found to be allelic with these two mutations. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
