Selection of Citrus limon cell culture variants resistant to the mal secco toxin |
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| Institution: | 1. ICAR-Central Plantation Crops Research Institute (ICAR-CPCRI), Kasaragod, India;2. ICAR-Indian Agricultural Research Institute (ICAR-IARI), New Delhi, India;1. State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, P.R. China;2. University of the Chinese Academy of Sciences, Beijing 100039, P. R. China;3. Longrun Pu-er Tea Academy, Yunnan Agricultural University, Kunming 650201, P. R. China;4. College of Pharmacy, Anhui University of Traditional Chinese Medicine, Hefei 230038, P. R. China;1. Aortic Institute at Yale-New Haven Hospital, Yale University School of Medicine, New Haven, Connecticut;2. Pediatric Heart Center, Skanes University Hospital, Department of Clinical Sciences, Lund University, Sweden;3. Department of Pediatrics (Cardiology), Yale University School of Medicine, New Haven, CT;4. Department of Political Science and Economics, Rowan University, Glassboro, New Jersey;5. Department of Economics and Department of Preventive Medicine, Stony Brook University, Stony Brook, New York;6. Department of Cardiovascular and Endovascular Surgery, Kazan State Medical University, Kazan, Russia |
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| Abstract: | Embryogenic cell cultures of Villafranca lemon capable of growth were selected in the presence of toxin produced by the fungus Phoma tracheiphila, the causal agent of Mal secco. Mal secco is a serious tracheomycotic disease of lemon (Citrus limon Burm. f.) and citron (C. medica L.). Rigorous clonal selection with the variant line was conducted for six consecutive subcultures. Viability of cell lines was monitored by the use of fluorescein diacetate. The stability of the resistance was examined after growth on non-selective medium for three subcultures. The variant line Var. 1.117 showed stable resistance. Cells of the resistant variant line maintained their enbryogenic capacity. Callus induced from the resulting somatic embryos also displayed resistance to the toxin. |
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