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Isolation of galactinol from leaves of Cucumis sativus
Affiliation:1. Department of Pharmacology, Xuan Wu Hospital of Capital Medical University, Beijing Geriatric Medical Research Center, Key Laboratory for Neurodegenerative Diseases of Ministry of Education, 45 Changchun Street, Beijing 100053, PR China;2. School of Pharmaceutical Sciences, Zhengzhou University, 100 Science Avenue, Zhengzhou, Henan Province 450001, PR China;3. Institute of Clinical Pharmacology, Guangzhou University of Chinese Medicine, 12 Jichang Road, Guangzhou, Guangdong Province 510405, PR China
Abstract:A simple procedure for the isolation of galactinol from leaves of Cucumimis sativus L. has been developed. The procedure yielded approx. 60% of the galactinol originally present in leaves with an apparent purity of 97%. Gas chromatographic and mass spectral analysis indicated that the compound was identical to galactinol isolated from sugar beet. The cucumber leaf galactinol was found to be suitable as a substrate for a galactosyl transferase enzyme which catalyses the formation of stachyose.
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