A new solid medium for the isolation and enumeration of Thiobacillus ferrooxidans and acidophilic heterotrophic bacteria |
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| Affiliation: | 1. Radboud University, Institute for Molecules and Materials, Heyendaalseweg 135, Nijmegen 6525AJ, The Netherlands;2. ASV Venlo, Laurentiusstraat 90, 5921GM Venlo, The Netherlands;1. Centro de Biotecnología, Universidad Católica del Norte, Av. Angamos 0610, Antofagasta, Chile;2. Centro de Investigación en Estudios Avanzados del Maule (CIEAM), Universidad Católica del Maule, Av. San Miguel 3605, Talca, Chile;3. Département de Chimie Analytique, Université Claude Bernard Lyon1, 43, boulevard du 11 Novembre 1918, 69100 Villeurbanne, France;4. Departamento de Matemáticas, Facultad de Ciencias, Universidad Católica del Norte, Av. Angamos 0610, Antofagasta, Chile;1. Université Paris Descartes et Assistance Publique-Hôpitaux de Paris, Hôpital Cochin, Service de Virologie, Paris, France;2. Assistance Publique-Hôpitaux de Paris, Hôpital Trousseau, Service de Virologie, Paris, France;3. CH de Versailles, Service de Biologie, Unité de Microbiologie, Le Chesnay, France;4. CHU Clermont-Ferrand, Laboratoire de Virologie, Centre National de Référence des Entérovirus-Parechovirus- Laboratoire associé, Clermont-Ferrand, France;5. Université d''Auvergne, EA4843 « Epidémiologie et Pathogénie des Infections à Entérovirus », Faculté de Médecine, Clermont-Ferrand, France;6. Hospices Civils de Lyon, Laboratoire de Virologie, Centre National de Référence des Entérovirus-Parechovirus, Lyon, France;1. Environmental Microbiology, Institute of Biosciences, TU Bergakademie Freiberg, Leipziger Str.29, 09599 Freiberg, Germany;2. Departamento de Biología, Faculta de Química y Biología, Universidad de Santiago de Chile, Av. Libertador Bernardo O’Higgins 3363, 9160000 Santiago, Estación Central, Chile;3. College of Environmental Science and Engineering, Donghua University, Songjiang, Shanghai, PR China;1. Department of Civil and Environmental Engineering, Rice University, 6100 Main St., Houston, TX 77005, USA;2. Department of Civil and Environmental Engineering, University of Houston, 4800 Calhoun St., Houston, TX 77204-4003, USA |
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| Abstract: | A solid medium (FeTSB) was developed for the simultaneous isolation and enumeration of the iron-oxidising bacterium Thiobacilluls ferrooxidans and acidophilic heterotrophic bacteria. The medium consisted of ferrous sulfate, tryptone soya broth and basal salts, solidified with agarose, although bacteriological agar could be substituted for some strains. The medium has been used to isolate bacteria from natural environments and also in laboratory studies of characterised strains. The factors which influence the success of colony growth on solid media are discussed. |
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