The stimulation of GABAB receptors increases serotonin release in the rat suprachiasmatic area |
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| Institution: | 1. Departamento de Inmunología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, 04510, Mexico City, Mexico;2. Departamento de Parasitología, Instituto Nacional de Diagnóstico y Referencia Epidemiológicos, 01480, Mexico City, Mexico;3. Facultad de Medicina, Universidad Nacional Autónoma de México, 06726, Mexico City, Mexico;4. Departamento de Biología, Facultad de Química, Universidad Nacional Autónoma de México, 04510, Mexico City, Mexico;5. Unidad Periférica del Instituto de Investigaciones Biomédicas en el Instituto Nacional de Neurología y Neurocirugía, 14269, Mexico City, Mexico;6. The Institute of Physiology and Pathophysiology, Medical Faculty, Philipps University, D-35037, Marburg, Germany;7. Stroke Clinic, Instituto Nacional de Neurología y Neurocirugía, 14269, Mexico City, Mexico;1. Department of Pharmacology, University of Minnesota, 6-120 Jackson Hall, 321 Church Street SE, Minneapolis, MN 55455, USA;2. Department of Neuroscience, University of Minnesota, 6-145 Jackson Hall, 321 Church Street SE, Minneapolis, MN 55455, USA;3. IDINE, Departmento de Ciencias Médicas, Facultad de Medicina, Universidad de Castilla La Mancha, C/Almansa 14, 02006, Albacete, Spain;1. Cognitive Neurology Research Group, University of Exeter Medical, School, College House, St Luke''s Campus, Exeter, UK;2. Human Cognitive Neuroscience, University of Edinburgh, 7 George Square, UK;3. Discipline of Psychology, University of Exeter, Washington Singer Laboratories, Exeter, UK;4. Department of Psychology, Heriot-Watt University, Edinburgh, UK;1. College of Forensic Science, School of Medicine, Xi''an Jiaotong University, No. 76, Yanta West Road, Xi''an, Shaanxi 710061, China;2. Xi’an Mental Health Center, Xi’an, Shannxi, China;3. Key Laboratory of Environment and Genes Related to Diseases, Ministry of Education, Xi''an, Shaanxi 710061, China |
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| Abstract: | The action of γ-aminobutyric acid (GABA) and related compounds on the spontaneous release of newly synthesized 3H]5-hydroxytryptamine (3H]5-HT) was studied in the suprachiasmatic area (SCA) using a superfusion system. GABA (10 μM) increased 3H]5-HT release from SCA by up to 190%. Bicuculline or picrotoxin (10 μM) failed to inhibit the stimulatory effect of GABA. Muscimol (10 μM), a GABAA agonist, was ineffective, however β-p-chlorophenyl GABA, R(?)baclofen, enhanced over 200% the release of the indoleamine; this latter effect was stereospecific. RS baclofen was twice less potent than the R(?)isomer in increasing the 3H]5-HT release. S(+)baclofen failed to affect the release of the indoleamine, whereas it attenuated the effect of its enantiomer. The effect of R(?)baclofen was Ca2+ dependent and was abolished by tetrodotoxin (TTX).Taken together these results suggest that in the SCA, 3H]5-HT release is facilitated by the stimulation of GABAB receptors. The possible localization of these receptors is discussed in the light of morphological data recently reported by Bosler et al. (1985) and results obtained after TTX application. |
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