Isolated microspore derived plant formation via embryogenesis in Triticum aestivum L. |
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| Affiliation: | 1. Human Informatics Research Institute, National Institute of Advanced Industrial Science and Technology, Central 6, 1-1-1 Higashi, Tsukuba, Ibaraki, Japan;2. Research Institute of Energy Frontier, National Institute of Advanced Industrial Science and Technology, 16-1 Onogawa, Tsukuba, Ibaraki, Japan;1. College of Physics & Materials Science, Tianjin Normal University, Tianjin 300387, People''s Republic of China;2. School of Science, Tianjin Polytechnic University, Tianjin 300387, People''s Republic of China;3. Tianjin International Joint Research Centre of Surface Technology for Energy Storage Materials, Tianjin Normal University, Tianjin 300387, People''s Republic of China |
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| Abstract: | Embryogenesis in isolated microspores of wheat (Triticum aestivum L.) leading to plant regeneration has been established on modified liquid N6 medium (supplemented with 2,4-D, casein hydrolysate and Ficoll). Globular embryoids which were obtained after 6–8 weeks of culture of competent embryogenic microspores produced perfect embryoids when transferred to regeneration medium. Embryoids were differentiated to plants on other modified N6 agar medium (0.75% w/v agar, 20 g/l sucrose, 1 g/l myo-inositol, 8.8 μM 6-benzylaminopurine (BAP), 11.4 μM indoleacetic acid (IAA), 160 mg/l glutamine, 10 mg/l proline). Responses of microspores in regeneration and embryoid differentiation varied depending on the constituents of the media and genotypes used. |
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