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Analysis of lipids and fatty acids during the germination of Brassica campestris cv. esculenta seeds
Institution:1. Institute of Applied Geosciences, Technical University Darmstadt, Schnittspahnstr. 9, D-64287 Darmstadt, Germany;2. Chemical and Biological Working Environment, National Institute of Occupational Health, Gydas Vei 8, PO Box 8149 Dep, N-0033 Oslo, Norway;3. National Research Centre for the Working Environment, DK-2100 Copenhagen, Denmark;4. Department of Arctic Technology, University Centre in Svalbard (UNIS), N-9171, Longyearbyen, Svalbard, Norway;5. Department of Chemistry, Biotechnology and Food Sciences (IKBM), Norwegian University of Life Sciences (NMBU), N-1432 Ås, Norway;1. College of Pharmacy, Chungnam National University, Daejeon 34134, South Korea;2. College of Agriculture and Life Sciences, Chungnam National University, Daejeon 34134, South Korea;3. Faculty of Agricultural Sciences, University of Gezira, Wad Medani, Sudan;1. Mass Spectrometry Centre, Department of Chemistry & QOPNA, University of Aveiro, Campus Universitário de Santiago, 3810-193, Aveiro, Portugal;2. Thermo Fisher Scientific, Hanna-Kunath-Straße 11, 28199, Bremen, Germany;3. Institute of Bioanalytical Chemistry, Faculty of Chemistry and Mineralogy, Universität Leipzig, Germany;4. Center for Biotechnology and Biomedicine, Universität Leipzig, Germany;5. Department of Chemistry & CESAM, University of Aveiro, Campus Universitário de Santiago, 3810-193, Aveiro, Portugal;1. Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, 818 South Beijing Road, Urumqi, Xinjiang, 830011, China;2. University of Chinese Academy of Sciences, 19 Yuquan Road, Shijingshan, Beijing, 100049, China;3. School of Agriculture and Environment, Faculty of Science, The University of Western Australia, 35 Stirling Highway, Crawley, WA, 6009, Australia;4. Department of Primary Industries and Regional Development of Western Australia, 3 Baron-Hay Court, South Perth, WA, 6051, Australia;5. School of Veterinary and Life Sciences, Murdoch University, Australia;1. INRA, UMR408 Sécurité et Qualité des Produits d’Origine Végétale, F-84000 Avignon, France;2. Université d’Avignon et des Pays du Vaucluse, UMR408 Sécurité et Qualité des Produits d’Origine Végétale, F-84000 Avignon, France;1. Xi’ an Botanical Garden of Shaanxi Province, Institute of Botany of Shaanxi Province, Xi’an 710061, PR China;2. State Key Laboratory of Grassland Agro-ecosystems, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou 730000, PR China
Abstract:Turnip tops seeds have a high lipid content (47.22% dry wt.); there is clear predominance of neutral lipids, mainly triglycerides, which represent 71.8% of the total lipid content.These triglycerides decrease during germination, with a maximum descent taking place between the 5th and 6th days of germination; this coincides with the maximum content in fatty acids in the seeds. However, phospholipids and glycolipids increase gradually during the same period. Gas-chromatography studies of the total and free fatty acids of these seeds reveals a predominance of those with an even number of carbon atoms; the proportion of unsaturated fatty acids is greater than that of the saturated kind. Among the former, of note are the high proportions mainly of erucic acid and oleic acid present in many seeds of the Cruciferae; the main saturated fatty acids found are palmitic, stearic and behenic acid.
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