Receptor-mediated degradation of insulin in isolated rat adipocytes: Formation of a degradation product slightly smaller than insulin |
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Institution: | 1. Molecular Recognition Materials Research Unit, Nanotec-PSU Center of Excellence on Drug Delivery System, Department of Pharmaceutical Chemistry, Faculty of Pharmaceutical Sciences, Thailand;2. Department of Anatomy, Hatyai, Songkhla 90112, Thailand;3. Animal House Division, Faculty of Science, Prince of Songkla University, Hatyai, Songkhla 90112, Thailand;4. National Nanotechnology Center (NANOTEC), National Science and Technology Development Agency (NSTDA),Thailand Science Park, Phahonyothin Road, Pathum Thani 12120, Thailand |
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Abstract: | More than 90% of the radioactivity associated with isolated rat adipocytes incubated with TyrA14-125I]monoiodoinsulin represented at steady state iodoinsulin possessing full binding affinity. In contrast, about half of the radioactivity dissociating from the cells was 125I]monoiodotyrosine. The other half was of a molecular size similar to that of iodoinsulin as judged from gel-filtration chromatography. However, the descending limb of the ‘insulin’ peak (i.e., the smaller molecules) possessed a reduced binding activity compared with native iodoinsulin, material from the ascending limb, or a similar fraction isolated from dissociation medium from IM-9 lymphocytes, a cell type devoid of receptor-mediated insulin degradation. The cells, thus, release an intermediary degradation product. |
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