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Esterase activity and isozymes during the ontogeny of stamens of male fertile lycopersicon esculentum mill., A male sterile stamenless-2 mutant and the low temperature-reverted mutant
Institution:1. Bioneer-FARMA, Department of Pharmacy, Universitetsparken 2, Copenhagen DK-2100, Denmark;2. Section of Pharmaceutical Design and Drug Delivery, Department of Pharmacy, University of Copenhagen, Universitetsparken 2, Copenhagen DK-2100, Denmark;1. National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan 430070, China;2. College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China;3. College of Plant Science & Technology, Huazhong Agricultural University, Wuhan 430070, China;4. Section of Cell and Developmental Biology, University of California San Diego, La Jolla, CA 92093-0116, USA;1. Drug Delivery and Disposition, KU Leuven, Gasthuisberg O&N II, Herestraat 49 – box 921, 3000 Leuven, Belgium;2. Division of BioAnalytical Chemistry, Vrije Universiteit Amsterdam, Amsterdam, The Netherlands;1. Newcastle University Centre for Cancer, Newcastle University, Newcastle upon Tyne, UK;2. Department of Pharmaceutical and Medical Chemistry, University of Münster, Münster, Germany;3. Division of Cancer Sciences, University of Manchester, Manchester, UK;4. Royal Manchester Children''s Hospital, Manchester, UK;5. Great North Children''s Hospital, Newcastle, UK;6. Pharmacy Department, Alder Hey Children''s NHS Foundation Trust, Liverpool, UK;1. Research Division, Central Texas Veterans Health Care System, Temple, TX, USA;2. Division of Pharmacology and Toxicology, College of Pharmacy, The University of Texas at Austin, Austin, TX, USA;3. Department of Internal Medicine, Dell Medical School, The University of Texas at Austin, Austin, TX, USA;1. Department of Civil, Construction, and Environmental Engineering, Iowa State University, Ames, IA 50011, USA;2. Department of Materials Science and Engineering, Iowa State University, Ames, IA 50011, USA
Abstract:A comparative study of the activity and isozymes of esterase during the ontogeny of stamens of normal male fertile (MF) Lycopersicon esculentum, a male sterile stamenless-2 (sl-2/sl-2) mutant (MS), and the low temperature reverted mutant was conducted. In MF and MS stamens, a major isozyme associated with tapetum development was present at early stages whereas another isozyme related to pollen formation was observed at later stages of development. There was a difference, however, in the timing of the appearance of these isozymes in MF and MS stamens. Also, the number and intensity of most bands, and the overall and specific activity of esterase was higher in MF than in MS stamens. The isozyme pattern, the number and intensity of bands and the overall activity of esterase was comparable in MF and MS-reverted stamens from low temperature grown plants. Male fertile stamens from low temperature-grown plants contained lower esterase activity than those grown in the greenhouse. The results are discussed with respect to the mechanism of male sterility in the sl-2/sl-2 mutant of tomato.
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