Screening methods to detect toxigenic fungi in liquid medium |
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| Institution: | 1. Program of Master in Science, Technology and Food Safety, Cesumar Institute of Science Technology and Innovation - ICETI. University Center of Mariná, UNICESUMAR, Maringá-PR, Brazil;2. Department of Animal Breeding and Nutrition, UNESP, College of Veterinary Medicine and Animal Sciences, São Paulo State University, Botucatu, Brazil;3. Department of Veterinary Science, UNICESUMAR, University Center of Maringá, Brazil;4. Department of Pharmacology and Therapeutics, UEM, State University of Maringá, Maringá, Brazil;5. Department of Animal Science, UFS, Federal University of Sergipe, São Cristóvão, Brazil;6. Department of Animal Science, UEM, State University of Maringá, Maringá, Brazil |
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| Abstract: | Rapid and sensitive methods for identifying toxin production by toxigenic fungi cultivated in liquid medium were developed. The production of aflatoxins B1, B2, G1, G2, sterigmatocystin, ochratoxin A, patulin, penicillic acid, citrinin and zearalenone was detected employing thin layer chromatography and high performance liquid chromatography detecion with or without extraction and purification procedures. No significant variation was found and toxins could be detected after 2–4 days incubation. The sensitivity of the methods and recovery after extraction have been estimated. |
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