The response of anther culture to culture temperature varies with growth conditions of anther-donor plants

The response of anther culture to culture temperature was tested using anther from wheat (Triticum aestivumL.)(3 spring varieties and 1 semi-winter variety) grown under two different conditions, in the field in spring and summer and in the greenhouse in winter and spring in Beijing. The results showed that the most suitable culture temperature for anther culture of field-grown materials was about 2°C higher than that of the greenhouse-grown materials, no matter what genotype was used. For example, in anther culture of the variety Jinghong 5, the highest yield of green pollen plantlets appeared at a culture temperature of 30°C when the anther-donor plants had been grown in the greenhouse, but was at 32°C when the donor plants were grown in the field. It was noticed that the anther of the greenhouse-grown material did not develop as well as those of the field-grown material.