Substance P-related peptides in the hypothalamus of Amphibia |
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Authors: | Dr. Giovanni Gaudino Aldo Fasolo |
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Affiliation: | (1) Istituto di Anatomia comparata, Università di Torino, Via Giolitti, 34, I-10123 Torino, Italy |
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Abstract: | Summary The occurrence of Substance P-(SP)-related peptides in the hypothalamus of three species of Amphibia (newt, clawed, toad, frog) was studied immunohistochemically employing the indirect immunofluorescence method or a double-step technique (indirect immunofluorescence followed by the peroxidase-antiperoxidase complex method). SP-like immunopositive fibers are seen throughout the hypothalamus. They are especially abundant in the preoptic area and in the outer zone of the median eminence, suggesting a role of SP-related peptides in the hypothalamo-hypophysial regulation in these animals. Some SP-like neurons are seen in the posterior hypothalamus and in the preoptic area. In the newt, such SP-like immunopositive neurons occur frequently in the preoptic periventricular grey.Work performed under the C.N.R. project Biologia della Riproduzione |
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Keywords: | Substance P-related peptides Immunohistochemistry Hypothalamus Amphibia (Triturus cristatus, Xenopus laevis, Rana esculenta) |
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