| 透明颤菌血红蛋白基因在产PHB重组大肠杆菌中的引入 |
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| 引用本文： | 于慧敏,史悦,张延平,杨胜利,沈忠耀.透明颤菌血红蛋白基因在产PHB重组大肠杆菌中的引入[J].微生物学报,2001,41(5):548-552. |
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| 作者姓名： | 于慧敏 史悦 张延平 杨胜利 沈忠耀 |
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| 作者单位： | 1. 清华大学化工系生物化工研究所,
2. 中国科学院上海生物工程研究中心, |
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| 基金项目： | 本课题为国家“九五”攻关项目(96-03-03-02);国家自然科学基金重点项目(29834103);国家自然科学基金面上资助项目(29876021) |
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| 摘 要： | 将透明颤菌血红蛋白基因 (vgb)采用插入染色体的方式引入产聚 β 羟基丁酸酯(PHB)重组大肠杆菌VG1 (pTU1 4)中 ,以从分子水平上提高克隆菌对氧的利用率 ,解决PHB发酵生产过程中的供氧矛盾 ,透明颤菌血红蛋白的一氧化碳差光谱分析明表 ,vgb基因可以在VG1 (pTU1 4)中成功表达 ,且其表达量受溶氧水平的调控。Vgb基因的引入可以同时促进菌体生长和PHB产品的积累 ,且溶氧水平越低 ,VHB表达量越高 ,这种促进作用就越明显
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| 关 键 词： | 透明颤菌血红蛋白基因（vgb） 聚β-羟基丁酸酯（PHB） 溶氧调控 |
| 文章编号： | 0001-6209(2001)05-0548-05 |
INTRODUCTION OF VITREOSCILLA HEMOGLOBIN GENE IN A RECOMBINANT E. COLI FOR PHB PRODUCTION |
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| Yu Huimin,\ Shi Yue,\ Zhang Yanping,\ Yang Shengli,\ Shen Zhongyao.INTRODUCTION OF VITREOSCILLA HEMOGLOBIN GENE IN A RECOMBINANT E. COLI FOR PHB PRODUCTION[J].Acta Microbiologica Sinica,2001,41(5):548-552. |
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| Authors: | Yu Huimin \ Shi Yue \ Zhang Yanping \ Yang Shengli \ Shen Zhongyao |
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| Institution: | Department of Chemical Engineering, Institute of Biochemical Engineering, Tsinghua University, Beijing 100084, China. |
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| Abstract: | Vitreoscilla hemoglobin gene (vgb) was introduced into the recombinant Escherichia coli VG1(pTU14) for production of poly-beta-hydroxybutyrate (PHB), in order to enhance the oxygen uptake of strain from molecular level and to solve the problem of oxygen limitation in process of fermentation. The carbon monoxide difference spectra analysis of Vitreoscilla hemoglobin (VHb) showed that vgb could be successfully expressed in VG1 (pTU14), and this expression was under the regulation of dissolved oxygen (DO). The higher amounts of VHb could be got correspond to the lower level of DO. Because of the introduction of vgb, cell growth and PHB accumulation of VG1 (pTU14) were all strongly promoted during the process of batch culture. |
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