A model for lipase production by Candida rugosa |
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Authors: | P. Serra J. L. del Río J. Robusté M. Poch C. Solà A. Cheruy |
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Affiliation: | (1) Unitat d'Enginyeria Quimica, Universität Autònoma de Barcelona, 8193 Bellaterra, Barcelona, Spain;(2) St. Martin d'Heres, Grenoble, France |
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Abstract: | A model adequately describing the lipase production by Candida rugosa has been developed, calibrated and validated using new experimental data. Process modelling has been done using CAMBIO software (Computer Aided Modelling of BIOprocesses), allowing to easy and interactively test various hypothesis and reaction schemes.Olive oil, oleic acid and glycerol has been used as substrates. The model satisfactorily describes the time evolution of biomass growth as well as lipase production in all cases. In particular diauxic behavior is successfully characterized.Model development process has helped in obtaining a 3-fold increase in lipase production when using oleic acid as substrate instead of the original olive oil used.List of Symbols Oil g/l Oil concentration - Fa g/l Fatty acids concentration - Gly g/l Glycerol concentration - Cr g/l Biomass (dry weight) - Lp U/ml Lipase - p Oil hydrolysis rate - gly Uptake rate on glycerol - fa Uptake rate on fatty acids - lp Increase rate of lipase - Yca Biomass/Fatty acids yield - Ycg Biomass/Glycerol yield - Yla Lipase/Fatty acids yield - kl Specific growth rate on fatty acids - Kc Saturation constant - KI Inhibition constant for lipase - k11 Specific growth rate on glycerol - k3 Oil hydrolysis parameter |
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