The F1 genes of the F1F0 ATP synthase from the acidophilic bacterium Thiobacillus ferrooxidans complement Escherichia coli F1unc mutants |
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Authors: | Lynn D Brown Maureen E Dennehy Douglas E Rawlings |
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Institution: | Institute of Medical Microbiology, Technical University (RWTH) Aachen, Pauwelsstrasse 30, 52057 Aachen, FRG; National Institute of Public Health and Environmentral Protection, Bilthoven, the Netherlands |
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Abstract: | Abstract Because of the allelic variations within the M protein gene ( emm gene) of group A streptococci, reliable typing of this important human pathogen can be accomplished by the use of emm gene-specific oligonucleotide probes. Two technical modifications (a reverse dot blot and a reverse line blot hybridization assay) of a novel approach for the type-specific identification of emm genes have been developed. Both procedures involved amplification of an emm gene by polymerase chain reaction. The non-radioactively labeled amplicon was subsequently hybridized to a membrane carrying an array of immobilized emm gene-specific oligonucleotide probes, thus allowing the simultaneous analysis of the gene polymorphism in a single hybridization reaction. The feasibility of these rapid and easy to perform methods was shown for the unequivocal identification of reference strains and clinical isolates belonging to 16 different M serotypes. |
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Keywords: | Streptococcus pyogenes M protein Polymerase chain reaction Hybridization assay Gene polymorphism Epidemiology |
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