Induction of somatic embryogenesis and embryo development in Rumex acetosella L. |
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Authors: | Ljubinka ?ulafi? Sne?ana Budimir Radmila Vuji?i? Mirjana Ne?kovi? |
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Institution: | (1) Institute of Botany, Faculty of Science, University of Belgrade, Takovska 43, 11000 Belgrade, Yugoslavia;(2) Institute for Biological Research S. Stankovi, University of Belgrade, Takovska 43, 11000 Belgrade, Yugoslavia |
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Abstract: | Axillary buds of the dioecious plant Rumex acetosella L. were isolated and cultured in vitro. The callus tissue which developed at the basal parts of the explants displayed a high capacity for shoot formation. This morphogenetic pattern was predominant on Murashige and Skoog (MS) medium supplemented with 2% sucrose, 2.2 mgl-1 benzylaminopurine and 0.17 mgl-1 indole-3-acetic acid. Somatic embryogenesis was induced when the osmolality of the medium was increased by adding 6% sucrose instead of 2%, or hexitols in addition to 2% sucrose. Most of the embryogenic calli were formed on the basal parts of leaf laminae and bracts. Development and maturation was strongly promoted by transferring the tissue to a solid or liquid medium lacking benzylaminopurine and indole-3-acetic acid and supplemented with 10 mgl-1 gibberellic acid. The embryos germinated and developed into normal rosette plants when transferred to vermiculite moistened with hormone-free, half-strength MS salt solution. The histology of successive embryogenic stages is presented. |
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Keywords: | Rumex acetosella L somatic embryogenesis sucrose concentration gibberellic acid |
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