Coding sequence and flanking regions of the mouse vimentin gene |
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Authors: | Hartwig Hennekes Siegfried Kühn and Peter Traub |
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Institution: | (1) Max-Planck-Institut für Zellbiologie, Rosenhof, D-6802 Ladenburg bei Heidelberg, Germany |
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Abstract: | Summary Using a polyclonal antibody, a cDNA clone coding for part of mouse vimentin was identified in a gt11 expression library. DNA from this clone was used to screen a genomic library from Ehrlich Ascites Tumor cells for the mouse vimentin gene. A clone was found which contained the whole coding sequence and a large part of the 5 - and 3 -untranslated sequences. It was used to prepare a construct equivalent to a full-length cDNA clone. Extensive homologies to the vimentin sequence from other species were found for the coding and 3 -untranslated sequences and the promoter region. |
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Keywords: | Intermediate filament protein Vimentin Promoter |
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