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On-line determination of pigment composition and biomass in cultures of microalgae
Authors:Niels Thomas Eriksen  Jens Jørgen Lønsmann Iversen
Institution:(1) Institute of Biochemistry, Odense University, Campusvej 55, DK-5230 Odense M, Denmark
Abstract:Summary Rhodomonas sp. was grown in a photo-bioreactor equipped with a measuring cell in a spectrophotometer as part of an external flow loop. The apparent absorbance from 400 to 800 nm of the cell suspension was recorded at predetermined intervals and stored in a computer. From the spectra, the biomass and the concentrations of the two pigments chlorophyll a and phycoerythrin were determined in nitrogen-limited batch cultures.
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