Demonstration of endocytosis vesicles by means of electron-microscopic histochemistry |
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Authors: | Björn Sandström |
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Affiliation: | (1) Department of Human Anatomy, University of Uppsala, Uppsala, Sweden |
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Abstract: | Summary A phosphatase activity similar to that of the plasma membrane is demonstrated at the inside of the membrane, which delimits the lipid-containing lysosomes of chicken liver parenchymal cells. This is taken as a definite confirmation of their endocytotic origin.It should be possible to demonstrate endocytosis vesicles in other cells in this way. It is, however, pointed out, that cells with a weak plasma membrane activity may require particular attention to certain steps in the technical procedure.This study was supported in part by a grant from the Swedish Medical Research Council. |
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